standard multilayer 200 lab vibro screen price china
standard multilayer 200 lab vibro screen price china
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The plasma sputtering target is a standard size of 2 inches. Customers can choose different configurations of 1~3 target heads according to their needs to meet the different experimental needs of single-layer film or multilayer films of various materials.
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BAM Chapter 9: Vibrio | FDA
Members of the genus Vibrio are defined as Gram-negative, asporogenous rods that are straight or have a single, rigid curve. They are motile; most have a single polar flagellum, when grown in liquid medium. Most produce oxidase and catalase, and ferment glucose without producing gas (7). Three species, V. cholerae, V. parahaemolyticus, and V. vulnificus, are well-documented human pathogens (54,78,79, 90,101). V. mimicus (24,103,111), is a recognized pathogen (103) with similar characteristics to V. cholerae, except an ability to ferment sucrose. Other species within the genus, such as V. alginolyticus (51), V. fluvialis (71), V. furnissii (15),V. metschnikovii (39,70), and V. hollisae (40) are occasional human pathogens (1,39, 96). Vibrio species account for a significant proportion of human infections from the consumption of raw or undercooked shellfish (96). A Florida study of illnesses from raw shellfish consumption reported the following species in descending order of frequency;... See full list on fda.gov V. cholerae (6), the type species of the genus Vibrio, is the causative agent of cholera outbreaks and epidemics (34,54,126). Various biochemical properties and antigenic types characterize it. It can be differentiated from other Vibrio species, except V. mimicus, because its obligate requirement for sodium ion (Na+) (6) can be satisfied by the trace amounts present in most media constituents. Cholera enterotoxin (CT) is the primary virulence factor of the disease cholera. A genetic pathogenicity island designated VPI (vibrio pathogenicity island), which contains most genes necessary to cause cholera was demonstrated to regulate the CT gene (55). Most V. cholerae strains recovered from epidemic cholera cases contain a common somatic antigen and include serogroup O1 (54). Over 150 known somatic antigenic types have been identified. Strains that are agglutinable in Inaba or Ogawa serotypes of O1 antiserum are well-documented human pathogens. Until recently, only the O1 serogroup was a... See full list on fda.gov V. mimicus (24,102) has been associated with diarrhea following consumption of raw or undercooked seafood (96). Isolated from samples during a search for V. cholerae, V. mimicus can be differentiated from that closely related pathogen by sucrose nonfermentation. The organism will appear as green colonies on thiosulfate citrate bile salts sucrose (TCBS) agar and will grow in most common media without added NaCl. Virulence is poorly characterized, but some strains have been found to possess the cholera toxin gene (111), produce demonstrable CT in a tissue culture assay, and the ctxgene can be detected by PCR amplification. See full list on fda.gov V. parahaemolyticus (36,81), the leading cause of bacterial diarrhea associated with seafood consumption in Florida (64) and probably the US and occasionally causes septicemia (96). It is a halophilic estuarine organism found in coastal waters of virtually all temperate regions (27,52,101). In temperate regions, a seasonal occurrence in shellfish and in human infections has been reported, the majority in the warmer months of the year. In subtropical regions such as Florida, illness can occur year round. All strains share a common H antigen, but, to date, 12 O (symatic) types and over 70 K (capsular) antigens have been described, though many other strains are untypable (81,101). Most clinical isolates of V. parahaemolyticus are differentiable from environmental strains by their ability to produce a thermostable direct hemolysin (TDH), termed the Kanagawa phenomenon (82,120). The tdh gene has been cloned and sequenced (86,87). DNA probes now are available to test for this virulence ma... See full list on fda.gov V. vulnificus (33), the leading cause of death in the US related to seafood consumption and nearly always associated with raw Gulf Coast oysters (90,104), resembles V. parahaemolyticus on TCBS agar, but can be differentiated by several biochemical reactions, including β-galactosidase activity (31). Epidemiological and clinical investigations have shown that V. vulnificus causes septicemia and death following ingestion of seafood or after wound infections originating from the marine environment (43,118,129). Recent gene probe assays (29,134), PCR procedures (41), fatty acid profiles (68) and enzyme immunoassays (31,122) have been developed to detect and identify this pathogen. See full list on fda.gov The following species have also been isolated from human stools and/or from patients with gastroenteritis, with the consumption of shellfish as the predominant source of infection (96). V. metschnikovii differs from all other Vibrio species in lacking cytochrome oxidase (7). Some strains (biotype II) of V. fluvialis sp. nov. (now designated V. furnissii) produce gas during D-glucose fermentation (15). V. hollisae is a halophilic species that grows poorly, if at all, on TCBS agar which exhibits a delayed motility pattern (>48 hr) uncharacteristic of the other vibrios (7). A variant of the tdh gene virulence marker of pathogenic V. parahaemolyticus strains was detected in some V. hollisaestrains (40). See full list on fda.gov Table 1 presents the differential characteristics of the species most often associated with human illness related to seafood consumption. Tables can also be found in several publications, including Baumann and Schubert (7), Elliot et al. (31), McLaughlin (78) and West et al.(131). Table 1. Biochemical characteristics of human pathogenic Vibrionaceae commonly encountered in seafood* See full list on fda.gov V. cholerae V. cholerae O1 is excreted in great numbers in the feces of cholera patients and convalescents (34,54). The disease is transmitted primarily by the fecal-oral route, indirectly through contaminated water supplies (30,78,80,116,126,130). Direct person-to-person spread is not common. Food supplies may be contaminated by the use of human feces as fertilizer or by freshening vegetables for market with contaminated water (30,57,58,80,94). Cholera outbreaks in several countries and the US are thoug... V. parahaemolyticus This organism is frequently isolated from coastal waters and seafood in temperate zones throughout the world. It is the most frequent cause of foodborne disease in Japan (89), where many residents eat raw fish. A number of common-source gastroenteritis outbreaks attributed to V. parahaemolyticus have occurred in the US (57), associated with oyster consumption (88,96). Some foods implicated in the US are crab, shrimp, and lobster, which unlike fish in Japan, typically were cooked before eating... V. vulnificus The invasive species, V. vulnificus, the causative agent of septicemic shock (63,90,118), is a common organism in coastal waters of some areas of the US and other countries (60,90,122,124). It is reported to cause 20 to 40 U.S. cases each year of primary septicemia with a 50% mortality rate among individuals with liver disease and elevated serum iron levels (104). A review of cases has determined an association between septicemia and consumption of raw oysters, nearly all from Gulf Coast wate... See full list on fda.gov Vibrio species, like many other Gram-negative bacteria, grow in the presence of relatively high levels of bile salts. They are facultatively anaerobic and grow best under alkaline conditions. Isolation from foods is facilitated by the use of media formulated with an alkaline pH. Alkaline peptone water (APW) is used commonly for isolating several species of concern. The strict halophilic nature of V. parahaemolyticus probably accounts for the fact that illnesses caused by this organism were not documented in the US until workers began examining food and feces on appropriate media containing added salt. Media used for testing the biochemical reactions of V. parahaemolyticus should contain 2% or 3% NaCl. V. vulnificusrequires NaCl for growth. A minimum of 0.5% NaCl, the concentration of most prepared media, is adequate. Diluent used for transfer of cell suspensions or dilution preparation must contain NaCl; for example, phosphate buffered saline, PBS (31). TCBS agar (31) is a medium co... See full list on fda.gov Storage of Sample The sample should be cooled immediately after collection (about 7° C to 10° C) , then analyzed as soon as possible. Direct contact with ice should be avoided to maximize survival and recovery of vibrios. Vibrios can be injured by rapid cooling, but grow rapidly in seafood at ambient temperatures (20,21). Despite the recognized fragility of the vibrios to extremes of heat and cold, their survival is enhanced under mild refrigeration (13,14,16,38,50,95). When frozen storage of the sample is req... Genetic Based Techniques These newer technologies have the advantage of more rapid detection and identification and are included for those laboratories with the proper equipment. PCR-based identification offers a one-day analysis (5,8,9,35,41,66,67,107,109,119,125), while gene probe procedures, including those presented in this chapter, are one-to-two day analyses (29,37,42,61,69,76,77,87,97,100,133,134,136,137). The traditional qualitative procedure and the most probable number (MPN) technique require four-to-seven... Recommended Controls More than one plating medium should be used for vibrios because strains may vary in their growth characteristics. T1N3agar works well for all vibrios relevant to human health. Positive and negative control strains should be used for all phenotypic and genotypic assays to ensure appropriate interpretation of the reactions. See full list on fda.gov
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